Affinity chromatography of pig heart fumarase.
نویسندگان
چکیده
2-(5'-Phenylpentyl)fumaric acid was shown to be a competitive inhibitor (Ki 0.5 mM) of pig heart fumarase. After nitration of the aromatic ring, reduction to the amine and diazotization, the acid was attached via azo linkages to a Sepharose 4B-tyramine matrix. The resulting adsorbent was used for the affinity chromatography of crude fumarase, purifications of approx. 20-fold being obtained by specific elution with 0.01 M-citrate.
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عنوان ژورنال:
- The Biochemical journal
دوره 177 1 شماره
صفحات -
تاریخ انتشار 1979